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Guanidine thiocyanate CAS 593-84-0 is white crystalline powder, melting point of 120-122 ℃ (lit.). Stable,but light sensitive. Incompatible with acids (contact releases very toxic gas), strong oxidising agents. Guanidinium thiocyanate (GITC) is a chemical compound used as a general protein denaturant, being a chaotropic agent, although it is most commonly used in the extraction of DNA and RNA.
Appearance White crystal or crystalline powder
Solubility (35% in water) Clear and colorless
Assay, % ≥ 99 .5
NH4CI,% ≤ 0.1
Water, ,% ≤ 0.3
Ash, % ≤ 0.05
Melting point, ℃ 118-121
PH(11.8%IN WATER) 5-7
- Guanidinium thiocyanate (GITC) is a chemical compound used as a general protein denaturant, being a chaotropic agent, although it is most commonly used in the extraction of DNA and RNA.
- Guanidinium thiocyanate can be used to deactivate a virus, such as the influenza virus.
- Guanidinium thiocyanate is also used to lyse cells and virus particles in RNA and DNA extractions, where its function, in addition to its lysing action, is to prevent activity of RNase enzymes and DNase enzymes by denaturing them.